|Swaggerty, Christina - Christi|
|Kogut, Michael - Mike|
|Genovese, Kenneth - Ken|
|He, Louis - Haiqi|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2008
Publication Date: 1/10/2009
Citation: Li, X., Swaggerty, C.L., Kogut, M.H., Chiang, H., Ying, W., Genovese, K.J., He, H., Pevzner, I., Zhou, H. 2009. Genome-wide gene expression profiling of chicken spleen responding to Campylobacter jejuni infection in broilers [abstract]. Plant & Animal Genome XVII Conference, January 10-14, 2009, San Diego, California. p. 511.
Technical Abstract: Campylobacter jejuni (C. jejuni) is a zoonotic pathogen that causes human diarrhea worldwide. Chickens are a natural reservoir of C. jejuni. Understanding the host response to C. jejuni infection at the molecular level will lay the foundation to control human campylobacterosis by reducing food contamination. Two distinct genetic lines, resistant (line A) and susceptible (line B) to C. jejuni colonization, were utilized to profile the host response to C. jejuni infection using an Agilent chicken 44K microarray. Day-old chickens were challenged orally with C. jejuni and spleens collected for total RNA 7 days post-challenge. Twenty infected samples with highest (a) or lowest bacterial number (b) in cecal content and twenty non-infected (c) in each line were randomly pooled into four biological replicates. The pair comparisons among these three groups within each line were analyzed. The signal intensity of each gene was normalized using LOWESS method and a mixed model was used to identify differentially expressed genes by SAS (P less than 0.001). This was opposite to previous cecal tonsil microarray result. There were 468, 743, and 939 genes differentially expressed between groups a and c, groups a and b, and groups b and c in line A, respectively, and 201, 37, 126 genes in line B, respectively. More differentially expressed genes in spleen in line A than in line B were found. The results indicated that significantly different response to C. jejuni infection occurred between resistant and susceptible chicken lines, and the effects of interaction between genetics and tissue should be considered.