Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/1/2008
Publication Date: 10/28/2008
Citation: Germerodt, M., Beuermann, C., Rohrer, G.A., Snelling, W.M., Brenig, B., Knorr, C. 2008. Characterization and linkage mapping of 15 porcine STS markers to fine-map chromosomal regions associated with hernia inguinalis/scrotalis. Animal Genetics 39:671-672.
Interpretive Summary: Candidate genes for the genetic defect hernia inguinalis were selected in an attempt to develop markers that could be used for marker-assisted breeding to lower the incidence of this defect. Nine of the 15 markers developed were located in regions of the genome thought to be affecting the disease on pig chromosomes 3, 6 and 12. These markers will be useful to identify causative mutations and develop genetic markers that could be used by the swine industry. The markers were similarly useful in pigs of European and Chinese breeds among the samples of the DNA repository tested.
Technical Abstract: Candidate genes for the genetic defect hernia inguinalis were selected and microsatellite markers developed from PAC clones using the targeted oligonucleotide-mediated microsatellite identification (TOMMI) approach. Four markers (S0894, S0898, S0899 and S0903) were either uninformative or could not be reliably genotyped on the MARC panel under standard conditions. RH mapping of the genes used for PAC library screening assigned S0894, S0898 and S0903 to SSC6 and S0899 to SSC7. Microsatellites located in regions associated with hernia inguinalis in pigs were S0891, S0892, S0902, S0904 and S0905 on SSC12, markers S0895, S0896 and S0901 on SSC6 and S0893 on SSC3. These markers will be useful to identify causative mutations. S0897 mapped to SSC13 and S0900 mapped to SSC9. Distributions of alleles between European and Chinese breeds were similar; however, alleles found only in one breed were preferentially discovered in Chinese breeds amongst the samples of the DNA repository.