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ARS Home » Midwest Area » Madison, Wisconsin » Cereal Crops Research » Research » Publications at this Location » Publication #230425
Title: Barley and oat beta-glucan content measured by calcofluor fluorescence in a microplate assay

Author
item Schmitt, Mark
item Wise, Mitchell

Submitted to: North American Barley Research Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 8/18/2008
Publication Date: 10/26/2008
Citation: Schmitt, M., Wise, M.L. 2008. Barley and Oat Beta-Glucan Content Measured by Calcofluor Fluorescence in a Microplate Assay. North American Barley Research Workshop Proceedings, October 26-29, 2008, Madison, WI.

Interpretive Summary:

Technical Abstract: Beta-glucan levels in grains, particularly barley and oats, are receiving increased interest in part due to their recognized benefits to human health. While a number of methods to determine grain beta-glucan levels are available, each suffers from significant drawbacks for routine implementation. Adaptation of a Calcofluor fluorescence method to detection by a microplate fluorimeter results in a simple mechanism for measuring beta-glucan, using inexpensive reagents, and affordable, readily available instrumentation.