Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2007
Publication Date: 4/1/2007
Citation: Gordon, W.E., Sy, J., Zurakowski, D., Griffin, D., Jefferson, L., Castillo, L. 2007. Methionine requirements in healthy adolescents [abstract]. Journal of Federation of American Societies for Experimental Biology. 21(5):A334-A335. Interpretive Summary:
Technical Abstract: To investigate methionine requirements in healthy adolescents, we conducted an experiment in 28 children age 15.6+2.1 years, wt. 57.8+9.7 kg, using the intravenous indicator amino acid oxidation and balance (IV-IAAOB) technique. Children were randomly assigned to 7 different levels of methionine intake and received an L-amino acid diet providing the test methionine and adequate protein and energy intake for 3 days. On day 3, they received a primed, continuous, 3 hour fast, 5 hour fed intravenous infusion of L-[1-13C] leucine. Blood samples were obtained for isotopic enrichment of [13C] KIC and 13CO2. Preliminary data for 18 patients is presented. There was no difference in leucine balance at methionine intakes of 7, 14, or 21 mg. Leucine balance at methionine intakes of 7 and 14 mg were lower (P<0.01) when compared to intakes of 25, 29, 36, and 43 mg. There was a trend toward a difference (P<0.06) on leucine balance at methionine intakes of 21 and 25 mg. Leucine oxidation was lower (P<0.01) at methionine intakes of 25, 29, 36, and 43 mg.kg–1.d–1 than at 7 and 14 mg.kg–1.d–1. In this preliminary data, there was a trend to achieve leucine balance at methionine intakes of 25 mg.kg–1.d–1.