|Smith, C Wayne|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 6/1/2008
Publication Date: 6/1/2008
Citation: Brake, D., Smith, W.C. 2008. Flow cytometry on the stromal–vascular fraction of white adipose tissue. In: Yang, K., editors. Adipose Tissue Protocols. Methods in Molecular Biology Series. Second Edition. Totowa, NJ: Humana Press. 456: 221-229. Interpretive Summary:
Technical Abstract: Adipose tissue contains cell types other than adipocytes that may contribute to complications linked to obesity. For example, macrophages have been shown to infiltrate adipose tissue in response to a high-fat diet. Isolation of the stromal-vascular fraction of adipose tissue allows one to use flow cytometry to analyze cell surface markers on leukocytes. Here, we present a technical approach to identify subsets of leukocytes that differentially express cell surface markers.