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Title: Relationships among bovine heat shock protein 70 genotype, forage type, and plasma concentrations of HSP-70.

item NABHAN, L
item LAMB, M
item REITER, S
item Brown, Michael
item BROWN, JR., H

Submitted to: American Society of Animal Science Southern Section Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2007
Publication Date: 2/5/2008
Citation: Nabhan, L., Lamb, M., Reiter, S., Okimoto, R., Brown, M.A., Brown, Jr., H., Rosenkrans, Jr., C. 2008. Relationships among bovine heat shock protein 70 genotype, forage type, and plasma concentrations of HSP-70 [abstract]. American Society of Animal Science Southern Section Meeting, February 4-5, 2008, Dallas, TX. p. 3. Available on-line:

Interpretive Summary: Abstract only.

Technical Abstract: The bovine HSP-70 gene is polymorphic, shows breed bias, and is related to milk production. Our objective was to determine if composite HSP-70 genotypes were related to plasma concentration of HSP-70. Genomic DNA and plasma samples were collected from 71 cows. The cows were Angus (n = 18; Bos taurus), Brahman (n = 24; Bos indicus), and Angus-Brahman reciprocal crosses (n = 29). Cows were maintained on either endophyte-infected toxic tall fescue (TF; Festuca arundinacea Schreb.) or common bermudagrass [CB; Cynodon dactylon (L.) Pers]. A 523 base segment was amplified via PCR using specific primers developed from GenBank accession number U09861, and eight single nucleotide polymorphisms (SNPs) were identified. Animals were assigned to one of three composite genotypes based on SNP allelic combinations. Plasma concentrations of HSP-70 were lower (P < 0.05) for cows grazing bermudagrass than those grazing fescue (5.4 vs. 8.5 ng/mL). An interaction (P < 0.05) between forage type and HSP-70 genotype affected plasma concentrations of HSP-70. When that interaction was evaluated, SNP 2087 accounted for most of the variation observed. Cows that were CG at SNP 2087 and grazing fescue had greater (P < 0.01) concentrations of HSP-70 than cows grazing bermudagrass or those having CC at SNP 2087 (22.4 vs. 5.3, 5.5, and 7.8 ng/mL; respectively for CG-TF, CC-CB, CG-CB, and CC-TF). Polymorphisms within the HSP-70 gene are related to plasma concentrations of HSP-70, interact with forage type, and may be useful in identifying cattle that are less susceptible to fescue toxicosis.