Submitted to: Fishery Bulletin
Publication Type: Peer reviewed journal
Publication Acceptance Date: 7/1/2008
Publication Date: 9/30/2008
Citation: Karlsson, S., Renshaw, M., Rexroad III, C.E., Gold, J. 2008. Microsatellite primers for red drum (Sciaenops ocellatus). Fishery Bulletin. 106:476-82. Interpretive Summary: In an effort to conduct population genetic analyses in red drum (Sciaenops ocellatus), we developed 101 genetic markers and tested them on 24 fish obtained from Galveston Bay, Texas. Our results demonstrate that along with previously developed genetic markers for red drum, these will be useful in a variety of applications including stock-structure analysis, monitoring and assessment of red drum stock enhancement, parentage analysis, and generation of a red drum genetic map.
Technical Abstract: In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101, nuclear-encoded microsatellites designed and developed from a red drum (Sciaenops ocellatus) genomic library. The 101 microsatellites (Genbank Accession Numbers EU015882-EU015982) were amplified successfully and used to genotype 24 red drum obtained from Galveston Bay, Texas. A total of 69 of the microsatellites had an uninterrupted (perfect) di-nucleotide motif, while 30 had an imperfect di-nucleotide motif; one microsatellite had an imperfect tetra-nucleotide motif, while one had an imperfect and compound motif. Sizes of the cloned alleles ranged from 84-252 base pairs. A 'blast' search of the Genbank database indicated that all of the primers and the cloned alleles were unique (i.e., not duplicated). Along with PCR primers for red drum microsatellites developed previously, these will be useful in a variety of applications including stock-structure analysis, monitoring and assessment of red drum stock enhancement, parentage analysis as employed in aquaculture, and generation of a red drum genetic map. A table of the 269 PCR primers developed for red drum may be found at <http://wfsc.tamu.edu/doc> under the file name 'PCR primers for red drum (Sciaenops ocellatus) microsatellites.'