Submitted to: Genbank
Publication Type: Germplasm Release
Publication Acceptance Date: 7/16/2008
Publication Date: 7/16/2008
Citation: Kamps, T.L., Ortega, V.M., Williams, N.R., Chamusco, K.C., Scully, B.T., Chase, C.D. 2008. Batch submission and release of genomic survey sequences (GSS) derived from leaf tissue of bermudagrass (Cynodon transvaalensis) accession PI 290995. GenBank, National Center of Biotechnology Information. GenBank Accession Nos. Fl107200 to Fl107236.
Technical Abstract: The genus Cynodon is a member of the Poaceae sub-family Chlorideae. Genetic diversity within the Cynodon genus is well established among the nine taxa that comprise the bermudagrasses. Favorable turf traits distinctive of C. transvaalensis, a diploid species commonly found in central parts of South Africa, are fine leaf texture, high shoot density, and dense sod formation. Difficulties in using morphological characteristics for cultivar verification and in turf grass breeding can be alleviated with the application of molecular markers. We constructed a library of genomic clones with DNA extracted from the diploid C. transvaalensis accession PI290905. Quality DNA sequences greater than 100 bp in length were obtained for a randomly selected set of 37 clones. Sequence analysis revealed 4 clones (ca. 11%) harbored microsatellite loci. Repeat motifs were dinucleotide, perfect (TG)n, imperfect (TG)n, compound perfect (GT)n(GA)n, and compound perfect (TC)n(AC)n. Only clones containing the compound perfect loci have sufficient flanking sequence for the design of PCR primer pairs. These 37 sequences are unique additions to the public database as no significant similarities were found by a BLASTn test against the 479 available Cynodon sp. GSS reads currently available (May 29, 2008) at the NCBI GenBank. In addition to the 2 clones which may be used as SSR markers for Cynodon and related grasses, these sequences have the potential for use in tracking genetic inheritance of PI290905 in intraspecific hybrids or, C. transvaalensis in the interspecific hybrid progeny resulting from crosses with C. dactylon.