Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 5/30/2008
Publication Date: 6/18/2008
Citation: Yeh, H., Klesius, P.H. 2008. Molecular Cloning and Sequence of Channel Catfish (Ictalurus punctatus, Rafinesque 1818) Cathepsin S gene. Genbank. Acession No. EU732711.
Technical Abstract: Cathepsin S is a lysosomal cysteine endopeptidase of the papain family. This enzyme digests the invariant chain molecules so that antigenic peptides are able to load on the class II-associated invariant chain peptide of MHC. The complexes can subsequently be presented to the CD4 cell surface. In this communication, the channel catfish, Ictalurus punctatus, cathepsin S gene was cloned and analyzed. Total RNA from tissues was isolated and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RACE primers were used to PCR amplify 5’- and 3’-ends of the cathepsin S transcript. The complete channel catfish cathepsin S cDNA comprised 1530 bp including a 96-bp 5’-untranslated, a 990-bp open reading frame and a 444-bp 3’-untranslated regions. The open reading frame encodes a putative protein of 329 amino acid residues with calculated molecular mass (without potential glycosylation) of 36.7 kDa and pI of 5.96.