Submitted to: UJNR Food & Agricultural Panel Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 7/8/2008
Publication Date: 8/23/2008
Citation: Hou, C.T., Kim, H. 2008. Production of 7,10-dihydroxy-8(E)-octadecenoic acid from triolein via lipase induction by Pseudomonas aeruginosa PR3. United States-Japan Natural Resources, Food & Agricultural Panel. p. 34-35.
Technical Abstract: Hydroxy fatty acids (HFA) have gained important attention due to special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. Pseudomonas aeruginosa PR3 has been previously reported to produce mono-, di-, and tri-hydroxy fatty acids from different unsaturated fatty acids. Of those, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced with high yield from oleic acid by PR3. Until now, the substrates used for microbial HFA production were free fatty acids. However, if soybean oil (triacylglyceride) can serve as substrate for HFA production, it will save one step in the process to break it down to free fatty acids such as oleic acid. In this study triolein was used as a model substrate and it was initially reported that triolein could be efficiently utilized by PR3 to produce DOD. It seems that lipase activity was induced in PR3. Triolein was first hydrolyzed into oleic acid by the triolein-induced lipase and then the released oleic acid was converted to DOD by PR3. The optimum conditions for DOD production from triolein by PR3 were studied. Optimal initial medium pH and incubation temperature were pH 8.0 and 25oC, respectively. The magnesium ion is required for DOD production from triacylglycerol. Other important conditions including inoculums’ size, time for substrate addition, substrate concentration were also studied. Results from this study demonstrated that vegetable oils, without being intentionally hydrolyzed, could be used as efficient substrate for the microbial production of value-added hydroxy fatty acids.