Submitted to: Research in Veterinary Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/19/2009
Publication Date: 10/1/2009
Publication URL: http://ddr.nal.usda.gov/dspace/bitstream/10113/33713/1/IND44241121.pdf
Citation: Hoesing, L.M., Lehmkuhl, H.D., Cutlip, R.C. 2009. Minimum intravenous infectious dose of ovine progressive pneumonia virus (OPPV). Research in Veterinary Science. 87(2):329-331. Interpretive Summary: The minimum amount of ovine progressive pneumonia virus to infect a lamb was determined using an intravenous infection model. Since only 4 virions were required to infect a lamb intravenously, this establishes the i.v. route a sensitive infection model to test potential vaccines and therapeutics for OPPV.
Technical Abstract: The minimum intravenous infectious dose for ovine progressive pneumonia virus (OPPV) WLC1 was determined using twenty-four 6 month-old lambs. Twelve groups of two 6 month-old lambs were inoculated intravenously with tissue culture fluid containing ovine progressive pneumonia virus (OPPV) WLC1 titers starting at 10^7.6 TCID50 in ten-fold dilutions and were monitored for seroconversion using the OPPV agar gel immunodiffusion assay (AGID). Fifteen of the sixteen lambs given equal or greater than 10^0.6 TCID50 seroconverted, and virus could be isolated from peripheral blood leukocytes in thirteen out of the fifteen of these lambs. None of the eight lambs receiving less than 10^0.6 TCID50 seroconverted during the 12 months. The results of this study indicated that ~4 virions given i.v. were capable of establishing infection.