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Title: Increasing foliar Zn:Ni or Cu:Ni concentration ratios increase severity of nickel deficiency symptoms

item Wood, Bruce

Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/3/2009
Publication Date: 1/19/2010
Citation: Wood, B.W. 2010. Increasing foliar Zn:Ni or Cu:Ni concentration ratios increase severity of nickel deficiency symptoms. Acta Horticulturae. 868:163-169.

Interpretive Summary: The cause of an increase in incidence and severity of nickel deficiency in pecan orchards, and emerging incidence in woody perennial crops, is unknown. It was found that the relative concentration of Zn and/or Cu to Ni within plant tissues can regulate expression of Ni deficiency symptoms via an antagonistic impact on the biological availability of Ni for metabolic and physiological processes. This work identifies a likely causal agent for occurrence of Ni deficiency and presents evidence for the lack of sustainability of long-term fertilizer applications of Zn and Cu in agricultural systems.

Technical Abstract: The occurrence of nickel (Ni) deficiency of pecan [Carya illinoinensis (Wangenh.) K. Koch] in orchards is an increasingly common problem. There is uncertainty regarding the primary cause of the problem, as orchard soils have plenty of Ni. The influence of essential micronutrients on the endogenous bioavailability of Ni is unknown and is a possible factor triggering Ni deficiency. This study examines the linkage between Ni deficiency and endogenous foliar concentration of Ni, Zn, and Cu. This relationship was tested in a greenhouse study using ‘Desirable’ seedlings trees growing in an orchard soil known to cause Ni deficiency in potted trees. Amendment of the potting soil with various amounts of either Zn-sulfate or Cu-sulfate produced seedling trees possessing a variety of Zn:Ni and Cu:Ni concentration ratios in developing foliage, and growth/morphological symptoms expressing various degrees of Ni deficiency. Severity of Ni deficiency was unrelated to foliar Ni concentration, but strongly linked to foliar Zn:Ni or Cu:Ni ratios. Deficiency symptoms increased sigmoidally with increasing Zn:Ni or Cu:Ni ratio, and were correctable, regardless of the Zn:Ni or Cu:Ni ratio, in seedling trees by foliar applications of Ni-malate extracted from Alyssum biomass. Thus, Zn and Cu in foliage can function antagonistically to reduce the bioavailability of Ni and therefore trigger expression of Ni deficiency. Soil Zn or Cu supplements did not detectably affect foliar Ni concentration; thus, root uptake does not appear to be inhibited by Zn or Cu. It is concluded that Ni deficiency in pecan orchards is likely to be partially due to either Zn or Cu fertilization induced reductions in the physiological availability of Ni, perhaps via either competitive inhibition or sequestration. It is also concluded that long-term or excessive Zn and/or Cu fertilization of crops potentially triggers Ni deficiency through endogenous antagonisms; thus, potentially threatening the sustainability of commercial pecan enterprises.