Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 12/30/2003
Publication Date: 12/30/2003
Publication URL: http://www.ncbi.nlm.nih.gov
Citation: Guerrero, F. 2003. New gene coding regions from the horn fly, Haematobia irritans. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nlm.nih.gov. Assession numbers AI304277-AI304311, AI308661, CD492272-CD492329, CF350121-CF350204 Interpretive Summary: The horn fly, Haematobia irritans, is a blood-feeding parasite of cattle which is a major control problem for cattle producers. Pesticides are a primary means of controlling these flies, however, pesticide resistance is a developing problem for producers. With an aim of identifying genes which play a role in the development and maintenance of pesticide resistance in the horn fly, we have begun to isolate expressed gene sequences from the fly. We also have obtained sequences from larval stages of the fly which are in the diapausing state, the state which fly's use to overwinter. This is the first published collection of expressed gene sequences from the horn fly and we have obtained 214 sequences from two sources, pesticide resistant adult flies and diapausing fly larvae. These sequences have been submitted and published under accession numbers AI304277-AI304311, AI308661, CD492272-CD492329, CF350121-CF350240 in the National Center for Biotechnology Information Public Database.
Technical Abstract: We used an EST approach to isolate new gene coding regions from the horn fly, Haematobia irritans. Two sources of expressed gene sequences were utilized. First, a subtracted library was synthesized from adult mixed sex fly cDNA of an organophosphate and pyrethroid resistant population of flies subtracted with a susceptible Kerrville laboratory colony of flies and library clones sequenced. Second, a set of expressed genes isolated from a differential display experiment looking at diapausing versus non-diapausing larval expressed genes was sequenced. These sequences were used to search publicly accessible databases to identify sequence similarity to known genes and assign putative function to the horn fly genes. The 214 EST sequences have been submitted and published under accession numbers AI304277-AI304311, AI308661, CD492272-CD492329, CF350121-CF350240 in the National Center for Biotechnology Information Public Database.