|Day, James - Michael|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/10/2008
Publication Date: 6/1/2008
Citation: Zsak, L., Strother, K.O., Kisary, J., and Day, M. J. 2008. Partial genome sequence analysis of parvoviruses associated with enteric disease in poultry [abstract]. XII Parvovirus Workshop, Cordoba, Spain, June 1-5, 2008. Interpretive Summary:
Technical Abstract: Poult Enteritis Mortality Syndrome (PEMS) of turkeys and Runting-Stunting Syndrome (RSS) of chickens are significant viral enteric diseases of poultry. Although a number of different viruses, including avian reoviruses, rotaviruses, astroviruses and coronaviruses, have been isolated from the intestinal contents of birds in affected poultry flocks, their role in PEMS and RSS is not yet understood. Here, we report the application of a molecular screening method to detection of novel viruses in intestinal samples of chickens and turkeys exhibiting characteristic signs of enteric disease. The technique is based on random amplification of particle-associated nucleic acids in clinical samples. Using this method we successfully identified parvovirus DNA sequences in intestinal homogenates of affected birds. This is the first time partial genomic sequences of autonomous chicken and turkey parvoviruses have been described. Sequence analysis of the left end of the genome, including the complete nonstructural gene, demonstrated that the chicken and turkey parvoviruses were closely related to each other and representative of a novel member of the Parvovirus family. These parvoviruses may play a significant role in the etiology of PEMS and RSS.