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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #224946
Title: Studies on Dulcamara mottle virus Infectious Clone and Chimeric Genomes with Turnip yellow mosaic virus

Author
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item Martin, Robert
item DREHER, T - OREGON STATE UNIVERSITY

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/21/2008
Publication Date: 6/1/2008
Citation: Tzanetakis, I.E., Martin, R.R., Dreher, T.W. 2008. Studies on Dulcamara mottle virus Infectious Clone and Chimeric Genomes with Turnip yellow mosaic virus. Phytopathology. 98:S160.

Interpretive Summary:

Technical Abstract: The genus Tymovirus consists of viruses with positive sense, single-stranded capped RNA genomes that encode three open reading frames and terminate in a tRNA-like structure. An unpublished report of DuMV 3’ UTR indicated that DuMV seemed to terminate in an A-tail, the first such report for a tymovirus. We investigated the genomic 3’ UTR and determined that DuMV has an internal A-track near the terminus of the genome. The complete genome sequence was acquired and phylogenetic analysis showed distant relationships of DuMV with some of the tymoviruses infecting solanaceous hosts but did not reveal any evolutionary footprint that could give insight into the acquisition or evolution of the 3’ UTR. We developed an infectious clone of the virus to study the role of the unusual 3’ UTR and chimeras of Turnip yellow mosaic virus and DuMV with their heterologous 3’UTRs. One of the chimeras was able to replicate in planta, shedding light in the ability of tymoviral polymerases to recognize different features in 3’ UTRs and to support replication, indicating the feasibility of the acquisition of the DuMV 3’ UTR during a polymerase-mediated recombination event. These results show that recombination may be a driving force in the evolution of the genus.