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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #224939
Title: Strawberry Virus Survey in the United States and Canada

Author
item Martin, Robert
item TZNETAKIS, I - OREGON STATE UNIVERSITY

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/21/2008
Publication Date: 6/1/2008
Citation: Martin, R.R., Tznetakis, I.E. 2008. Strawberry Virus Survey in the United States and Canada. Phytopathology. 98:S99.

Interpretive Summary:

Technical Abstract: In an effort to determine the distribution of strawberry viruses in the United States and Canada, approximately 1500 samples were collected and either brought back or shipped to the USDA-ARS laboratory in Corvallis between 2002 and 2007. RNA was extracted from leaf tissue and archived at -80C for subsequent uses. During the same time, RT-PCR tests were developed for most known strawberry viruses. For this study 275 samples representing the major strawberry production areas in the United States and Canada were tested for: Beet pseudo yellows (BPYV), Fragaria chiloensis latent (FClLV), Strawberry crinkle (SCV), Strawberry latent ringspot (SLRSV), Strawberry mottle (SMoV), Strawberry mild yellow edge (SMYEV), Strawberry necrotic shock (SNSV), Strawberry pallidosis (SPaV) and Strawberry vein banding (SVBV) viruses, as well as a housekeeping gene as an internal control by RT-PCR. The Pacific Northwest had the highest rates of infection with the aphid-borne viruses but was virtually free of the whitefly transmitted viruses. In contrast, California, the southeastern United States, the northeastern United States and Ontario, and the Midwest had aphid and whitefly transmitted viruses in about equal numbers, with the Midwest having the lowest incidence of virus infection. BPYV was only found in the samples from California and the southeastern United States in these samples but has been detected from Maryland in previous studies. In the Pacific Northwest, fields with aphid control had very low incidence of virus infection compared to nearby fields without aphid control. Also the disease pressure was much lower in Oregon than in northern Washington or British Columbia.