Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2008
Publication Date: 4/11/2008
Citation: Shelby, K., Terenius, O., Popham, H.J. 2008. Preliminary analyses of the Heliothis virescens transcriptome [abstract]. Arthropod Genomics Symposium. p. 14.
Technical Abstract: Generation and analysis of genomic resources for the tobacco budworm, Heliothis virescens, are necessary for detailed functional genomic studies of the physiology and biochemistry of this highly destructive pest. In this study we present preliminary analyses of the ~45,000 publicly available H. virescens ESTs. ESTs were retrieved, assembled and annotated into 8074 contigs, and 7231 singletons; totaling 15,301 putative transcripts. 6497 of these have a significant BLAST to known homologs from other organisms, primarily insect species. Expression of selected immune response homologs in the fat body, midgut and hemocytes were monitored using real time PCR following bacterial and baculoviral infection. Results will be discussed.