Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 3/25/2008
Publication Date: 6/1/2008
Citation: Li, C., Feng, J., Ma, F., Vick, B.A., Jan, C.C. 2008. Identification of molecular markers linked to a new nuclear male-sterility gene ms7 in sunflower (Helianthus annuus L.). Proceedings of 17th International Sunflower Conference, June 8-12, 2008, Cordoba, Spain. p. 651-654. Interpretive Summary: Male sterility, including cytoplasmic male sterility (CMS) and nuclear male sterility (NMS), provide valuable approaches for hybrid breeding programs. In sunflower, CMS PET1 and the corresponding fertility restorers have been extensively used for almost all commercial hybrid production. To prevent cytoplasmic uniformity and reduce genetic vulnerability of sunflower hybrids, NMS has become a promising alternative system to CMS for hybrid development. Four unique NMS genes, designated ms6, ms7, ms8 and ms9, were identified from mutant HA 89 induced by mitomycin-C and streptomycin. The ms9 gene of NMS HA89-360 has been genetically mapped to linkage group 10 of the SSR genetic map, but ms7 of NMS remains unmapped since the release of NMS HA89-552. The objective of the present research was to identify molecular markers linked to the NMS ms7 gene in HA 89-552.
Technical Abstract: Nuclear male sterility (NMS) is an important alternative system to the cytoplasm male sterility (CMS) in hybrid breeding programs because of its stable male sterility and abundant available restorer resources. For sunflower (Helianthus annuus L.), NMS 89-552, a nuclear male-sterile mutant induced by mitomycin-C and streptomycin from an inbred maintainer line HA 89, possesses a single recessive gene, ms7, controlling male sterility. DNA markers linked to the ms7 gene were identified in a 93-plant F2 population derived from the cross of NMS 89-552 x RHA 271 by using simple sequence repeat (SSR), RFLP-derived sequence tagged site (STS), and target region amplification polymorphism (TRAP) markers. The ms7 gene was mapped to linkage group 6 of the public SSR genetic map. Four SSR markers (ORS349, ORS608, ORS1229 and ORS483), two RFLP-STS markers (STS8C4, STS9C1) and two TRAP markers (Tg3r165a-220, Tg3r165a-185) were located around the ms7 locus. The total genetic distance covered by those markers was 53.4 cM. SSR marker ORS608 and TRAP marker Tg3r165a-185 flanked the ms7 gene, at distances of 2.6 cM and 4.7 cM, respectively. The markers linked with the ms7 locus could be used to accelerate the male-sterile line breeding through marker-assisted selection technique.