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Title: Development of a novel assay for detection of Bluetongue virus in livestock: potential applications in animal health

Author
item Kato, Cecilia
item MAYER, RICHARD - 5410-10-00

Submitted to: Developments in Biologicals
Publication Type: Proceedings
Publication Acceptance Date: 10/23/2007
Publication Date: 10/23/2007
Citation: Kato, C.Y., Mayer, R.T. 2007. Development of a novel assay for detection of Bluetongue virus in livestock: potential applications in animal health. Developments in Biologicals. International Symposium on Animal Genomics for Animal Health, Paris, France, Oct. 23-25, 2007.

Interpretive Summary: Bluetongue virus (BTV) detection in livestock requires multiple steps for nucleic acid isolation, RNA transcription into DNA, and PCR amplification. We have developed a single tube assay for the detection of Bluetongue virus in pure solution, blood and insect homogenate. The assay is fast, simple, cost effective and does not require any enzymes.

Technical Abstract: Bluetongue virus (BTV) detection in livestock requires multiple steps for nucleic acid isolation, RNA transcription into DNA, and PCR amplification. Although the process has been streamlined and made portable through new instrumentation, current point of care application of BTV diagnostics still requires technical skill, costly equipment and reagents. We have developed a rapid, simple and cost effective method for BTV detection in blood to overcome these obstacles. Nucleic acid targets of interest can be discriminately identified within a complex solution, and no enzymatic amplification (reverse transcription or PCR) steps are necessary for signal detection. Current detection sensitivity is 1 amol in pure hybridization solution, 1 pmol in blood, and 1 fmol in insect homogenate.