Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2007
Publication Date: 3/31/2007
Citation: Lu,, S., Yu,, H., Chen,, S., Wang, X. 2007. Molecular and functional characterization of parasitism genes of the potato cyst nematode Globodera rostochiensis. Journal of Nematology. 39:79.
Technical Abstract: Potato cyst nematodes (Globodera rostochiensis and G. pallida) are quarantined pests threatening the potato industry in the United States. Secreted proteins encoded by parasitism genes expressed in the esophageal gland cells of plant-parasitic nematodes represent the primary molecules involved in plant parasitism. Putative parasitism genes including several CLE-like genes (GrCLEs), a chorismate mutase gene (GrCM-1), a venom allergen-like gene (GrVAP-1), a ubiquitin extension gene (GrUBI-1), an SKP-1-like gene (GrSKP-1), and three genes homologous, respectively, to 4D06, 7E05, and 33E05 genes of the soybean cyst nematode have been isolated from G. rostochiensis. In-situ mRNA hybridization showed the accumulation of their transcripts exclusively within the esophageal gland cells and Southern blot analysis confirmed the presence of these genes in the G. rostochiensis genome. RT-PCR and qRT-PCR analyses revealed that the expression of these genes was developmentally regulated. Currently, we are employing both in vitro and in vivo RNAi approaches to evaluate if these genes have a critical role in G. rostochiensis parasitism of plants.