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Title: Reconciliation of D-genome puroindoline allele designations with current DNA sequence data.

item Morris, Craig

Submitted to: Journal of Cereal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/13/2007
Publication Date: 8/20/2008
Citation: Morris, C.F., Bhave, M. 2007. Reconciliation of D-genome puroindoline allele designations with current DNA sequence data. Journal of Cereal Science 28:277-287.

Interpretive Summary: Kernel hardness (texture) is an important end-use quality trait in wheat. Variation in hardness is associated with variation in puroindoline genes. In this report, sequence data are reconciled with puroindolines allele nomenclature. Recommendations follow the International Wheat Genetics Symposium Catalogue of Gene Symbols.

Technical Abstract: Kernel texture is an important trait in cereals, especially wheat (Triticum sp.). Throughout the Triticeae, the puroindoline genes act to soften kernel endosperm. Absence or mutation of either or both of the two puroindolines, ‘a’ and ‘b’, in T. aestivum results in harder grain texture. Apparently only one puroindoline haplotype was contributed by the Aegilops tauschii variety that contributed the D-genome during allopolyploidation. Yet, world collections of Ae. tauschii exhibit a range of puroindoline sequence polymorphisms. Consequently, these genes, through synthetic hexaploids (x Aegilotriticum) can enrich the wheat gene pool. Lastly, the puroindolines represent a useful tool for phylogenetic analyses. Here we review original sequence data published and / or available in public databases to reconcile the known gene sequence polymorphisms with a systematic approach to the designating of puroindoline gene and allele symbols in T. aestivum, Ae. tauschii, and x Aegilotriticum. This system follows the recommendations adopted by the International Wheat Genetics Symposium and described in the Catalogue of Gene Symbols for Wheat. Errors, discrepancies and ambiguities in the puroindolines are reviewed; a reconciliation of all existing data is outlined.