Submitted to: Journal of Economic Entomology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 8/1/2010
Publication Date: 12/28/2010
Citation: Boykin, L.M., Shatters, R.G., Hall, D.G., Dean, D., Beerli, P. 2010. Genetic variation of Anastrepha suspensa (Diptera: Tephritidae) in Florida and the Caribbean using DNA microsatellite markers. Journal of Economic Entomology. 103(6):2214-2222. Interpretive Summary: There is gene/flow migration of A. suspensa between Florida and the Caribbean as demonstrated by MIGRATE and the Bayesian clustering programs STRUCTURE and TESS. The gene flow of this invasive insect is back and forth between Florida and Caribbean, and this movement is most likely attributed to the human movement of infested fruit. The data presented here and in Boykin et al. (2006) suggested that there is to genetic evidence for a host shift to citrus.
Technical Abstract: Anastrepha suspensa Loew, the Caribbean fruit fly, is indigenous to Florida and the Greater Antilles where it is an economic pest of fruit crops including citrus. Because of the geographic separation of many of its native locations and anecdotal descriptions of regional differences in host preferences, there have been questions about A. suspensa subspecies population structure. Seven DNA microsatellite markers were used to characterize the population genetic structure of A. Suspensa in Florida and the Caribbean from a variety of hosts including citrus. Seven hundred and twenty nine A. suspensa individuals from throughtout Florida, Puerto Rico, Cayman Island, Dominican Republic, and Jamaica were genotyped and displayed from 5 to 19 alleles for the seven loci, with expected heterozygosities ranging from 0.005 to 0.83. There were 5 unique alleles to Florida and 3 unique alleles in the Caribbean samples; however, no microsatellite alleles were specific to a single host plant. Furthermore, no population genetic structure was found using the Bayesian population genetic structuring programs STRUCTURE and TESS. Further analyses using MIGRATE revealed there is continuous gene flow between sampling sites in Florida and the Caribbean. These results support previous COI comparisons (Boykin et al., 2006) indicating there is no subspecies population structure that separates populations within and among Florida and Caribbean locations.