Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract only
Publication Acceptance Date: 1/31/2008
Publication Date: 4/15/2008
Publication URL: www.fasebj.org
Citation: Nielsen, F.H. 2008. A combined marginal deficiency of copper and zinc does not exacerbate oxidant stress asssociated with copper or zinc deficiency. [abstract] Journal of Federation of American Societies for Experimental Biology. 22:1103.1. Interpretive Summary:
Technical Abstract: Both copper deficiency (Cu-def) and zinc deficiency (Zn-def) result in oxidative stress. Thus, an experiment was conducted to determine whether a marginal Zn-def amplified oxidative stress responses to a marginal Cu-def, or vice versa. Weanling male Sprague-Dawley rats were assigned to groups of 10 and fed an AIN-93G diet with dried egg whites as the protein source and modified to increase oxidative stress (safflower oil instead of soybean oil and sucrose instead of dextrinized cornstarch) for 6 wk. Dietary variables were the basal diet containing (per kg) 1.5 mg Cu and 8 mg Zn, and basal diet supplemented (per kg) with 4.5 mg Cu, 10 mg Zn, or 4.5 mg Cu and 10 mg Zn. Two additional groups of 10 rats were fed the diets containing 18 mg Zn and 1.5 or 6 mg Cu per kg and pair-fed with corresponding rats fed 8 mg Zn/kg diet. Cu-def increased plasma extracellular superoxide dismutase and 8-isoprostaglandin F2a (F2-IsoP); the increases were amplified by pair feeding but not by Zn-def. Zn-def did not exacerbate the Cu def-induced decrease in plasma ceruloplasmin. Zn-def decreased urinary F2-IsoP in Cu-def but not Cu-adequate rats, and decreased plasma RANKL in Cu-adequate but not Cu-def rats. The dietary treatments did not affect urinary nitrate. The findings indicate that a combined marginal Cu-def and Zn-def did not exacerbate, and even alleviated, some signs (RANKL and F2-IsoP) of oxidative stress.