Submitted to: Animal Genetics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 8/9/2004
Publication Date: 12/1/2004
Citation: Serapion, J., Waldbieser, G.C., Wolters, W.R., Liu, Z. 2004. Development of Type I Markers in Channel Catfish Through Intron Sequencing. Animal Genetics 35:463-466. Interpretive Summary: The channel catfish genome can be compared with more completed genomes, such as zebrafish, in order to more efficiently identify genes and gene order. However, this comparative genomic approach requires the catfish genome map contain genes that can be identified in both species, and few of these genes have been placed on catfish maps. This research was designed to identify variable DNA sequences within portions of genes that do not encode proteins (a.k.a. introns), and DNA sequence was obtained for at least one intron from 42 genes. Eight genes contained a variable sequence in the intron, and six could be placed on the catfish genetic linkage map. The research demonstrated this approach to adding conserved genes to the catfish map was possible but at low efficiency.
Technical Abstract: Type I markers are useful for comparative gene mapping, but they have not been widely available in catfish. Here we took the approach of sequencing the intron sequences of genes with the assumption that the introns are more prone to mutations and may have higher chances of containing microsatellites. Primers were designed to amplify introns of 50 genes, of which 42 produced amplicons that were successfully sequenced. Eight genes contained a microsatellite sequence in the intron, and six were placed on the catfish genetic linkage map based on allelic inheritance in reference families.