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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #218828

Title: Leptospira Protein Expression During Infection

item Zuerner, Richard

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/2008
Publication Date: 1/20/2008
Citation: Zuerner, R.L. 2008. Leptospira Protein Expression During Infection [abstract]. Gordon Research Conference: Biology of Spirochetes. Poster No. 2-46.

Interpretive Summary:

Technical Abstract: We are characterizing protein expression in vivo during experimental leptospirosis using immunofluorescence microscopy. Coding regions for several proteins were identified through analysis of Leptospira interrogans serovar Copenhageni and L. borgpetersenii serovar Hardjo genomes. In addition, coding regions for several serovar Pomona genes were identified from selected cloned segments of the genome. Fusion proteins containing portions of these Leptospira proteins fused with poly-histidine tails were purified from Escherichia coli hosts and used to prepare specific antisera. Tissues obtained from experimentally infected hamsters were examined for the presence of bacteria using immunofluorescence microscopy. Bacteria were detected in liver, lung, and kidney and also spleen, pancreas, heart, reproductive tissue, and intestines. The distribution of antibody staining varied, depending on the protein target and tissue. In some organs the characteristic spirochete morphology is detectable, e.g. kidney, liver, and lung, while in other tissue, e.g. the red pulp of the spleen, bacterial antigens are predominantly localized in phagocytes. Current studies are focused at analyzing host protein expression by cells in close proximity to Leptospira to assess changes induced in the host during infection.