Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/29/2008
Publication Date: 4/9/2008
Citation: Chen, J., Lazarenko, O.P., Badger, T.M., Ronis, M.J. 2008. Wnt/RANKL-mediated bone growth promoting effects of blueberries in weanling rats [abstract]. The FASEB Journal. 22:314.2.
Technical Abstract: We studied the effects of dietary blueberry supplementation on bone growth in weanling rats. Weanling male and female rats were fed AIN-93G semi-purified diets supplemented with 10% whole blueberry powder for 14 and 30 days beginning on PND 21. In both sexes tibial bone mineral density and content and cortical bone thickness were all increased dramatically in blueberry-fed rats as compared to control casein AIN-93G fed animals (p < 0.05). The serum bone formation marker alkaline phosphatase (ALP) increased, but the resorption marker RatLaps decreased in blueberry-fed rats. Ex-vivo osteoclastogenesis and osteoblastogenesis assays and bone histomorphometric studies revealed decreased osteoclastogenesis and increased osteoblastogenesis in blueberry-fed rats compared to controls (p < 0.05). In contrast to control diets, gene expression from both bone and bone marrow of blueberry-fed rats showed dramatic decreases in RANKL and tartrate-resistant acid phosphate (TRAP) mRNAs but increases in bone formation markers ALP, osteocalcin, RUNX2, and the Wnt signaling related components Wnt5a, LRP5, LRP6 mRNAs. Beta-catenin protein level was increased in blueberry-fed rats in bone tissue relative to control rats (p < 0.05). These results indicate significant stimulation of bone growth associated with blueberry consumption in early development.