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United States Department of Agriculture

Agricultural Research Service

Title: Identification of SSR markers for a broad-spectrum blast resistance gene Pi-20(t) for marker-assisted breeding)

Author
item Li, Wei
item Lei, Cailin
item Cheng, Zhijun
item Jia, Yulin
item Huang, Dongyi
item Liu, Zhanling
item Wang, Jiulin
item Shi, Ke
item Zhang, Xin
item Su, Ning
item Guo, Xiuping
item Zhai, Huqu
item Wan, Jianmin

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/29/2008
Publication Date: 4/19/2008
Citation: Li, W., Lei, C., Cheng, Z., Jia, Y., Huang, D., Liu, Z., Wang, J., Shi, K., Zhang, X., Su, N., Guo, X., Zhai, H., Wan, J. 2008. Identification of SSR markers for a broad-spectrum blast resistance gene Pi-20(t) for marker-assisted breeding. Molecular Breeding. 22:141-149.

Interpretive Summary:

Technical Abstract: The Pi-20(t) gene in rice confers broad-spectrum resistance against diverse pathotypes (races) of Magnaporthe oryzae (formerly Magnaporthe grisea) in China. Two flanking, and three co-segregating SSR markers nearby the centromere region of chromosome 12 for Pi-20(t) were identified using 526 extremely susceptible F2 plants derived from a cross of Asominori, an extremely susceptible cultivar with a highly resistant cultivar, IR24. OSR32 was mapped at 0.2cM of Pi-20(t) at one side and RM28050 was mapped at 0.4cM of Pi-20(t) at another side. Three SSR markers (RM1337, RM5364 and RM7102) were found to co-segregate with Pi-20(t). RM1337 and RM5364 were found to be reliable markers to tag resistance conditioned by Pi-20(t) in a wide range of elite rice germplasm in China. Thus, these two SSR markers are useful in marker-assisted incorporation of Pi-20(t) into advanced breeding lines for achieving durable and broad spectra of resistance to M. oryaze.

Last Modified: 8/24/2016
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