Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/7/2007
Publication Date: 1/11/2008
Citation: Nimmakayala, P., Thomas, Y., Vajja, G., Cantrell, R., Kumar, P.S., Gibson, P., Levi, A., Reddy, U. 2008. Constructing an Initial Linkage Map for Ukrainian Melons. Plant and Animal Genome XVI Conference, P663.
Technical Abstract: High-quality melons have been developed in the Ukraines in the last 50 years using diverse melon germplasm. There is no or there is little information regarding genetic diversity in Ukrainian melons molecular markers. In this study, two Ukrainian melon lines “L22-1” (netted surfaced fruits with oval shape, yellow flesh colored fruits with the high sugar content and susceptible to powdery mildew) and “KRL” (smooth surfaced fruits with round shape, yellow green flesh color, low sugar content, and highly resistant to powdery mildew) were used for the development of an F2 mapping population. Ninety-two F2 plants derived from a single F1 plant were characterized for fruit qualities, including fruit weight, fruit length and diameter, fruit firmness and flesh thickness, soluble solids and sugar content. Transgressive segregation (whereas fruit qualities in some of the F2 progenies exceeded those of the parental lines) was observed for all fruit qualities examined. The F2 population is being used for the development of an initial linkage map based on Ukrainian melon lines. Over 400 polymorphic AFLP markers (EcoR I and Mse I) were developed and are being used for constructing an initial linkage map for the ‘Ukrainian Melon Project’, and for possible identification of quantitative trait loci (QTLs) linked to genes controlling melon fruit qualities. This linkage map will be merged with other maps developed so far for this important cucurbit crop.