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Title: Bio-functional Au/Si Nanrods for Pathogen Detection

item Park, Bosoon
item Siragusa, Gregory
item Lawrence, Kurt
item Windham, William

Submitted to: Proceedings of SPIE
Publication Type: Proceedings
Publication Acceptance Date: 9/11/2007
Publication Date: 11/30/2007
Citation: Park, B., Fu, J., Zhao, Y., Siragusa, G.R., Cho, Y., Lawrence, K.C., Windham, W.R. 2007. Bio-functional Au/Si Nanrods for Pathogen Detection. Proceedings of SPIE 6769-26.

Interpretive Summary: Since Salmonella is one of the major causes of bacterial gastroenteritis in humans and a source of many food-related outbreaks, the development of a rapid method for detecting Salmonella is necessary for microbiological analysis to control food safety. Several methods have been developed in order to detect the Salmonella pathogen; however, the biggest challenges are detection speed and sensitivity. Conventional microbiological techniques are time-consuming and labor-intensive for detecting foodborne pathogens. Bio-functionalized nanorod-based biosensor has shown great potentials for foodborne pathogenic bacteria detection. In this work, a novel biosensor was developed for Salmonella detection using nanotechnology.

Technical Abstract: Nanotechnology applications for food safety and biosecurity, especially development of nanoscale sensors for foodborne pathogen measurement are emerging. A novel bio-functional nanosensor for Salmonella detection was developed using hetero-nanorods. The silica nanorods were fabricated by glancing angle deposition method and the gold was sputtered onto the silica nanorods. Alexa488-succinimide dye was immobilized onto the annealed Si nanorods via the attachment between dye ester and primary amine group supplied by the 3-Aminopropyltriethoxysilane. The anti-Salmonella was conjugated to gold via Dithiobis[succinimidylpropionate] self-assembly monolayer. Due to the high aspect ratio nature of the Si nanorods, hundreds or thousands of dye molecules attached to the Si nanorods produced enhanced fluorescence signal. These biologically functionalized nanorods can be used to detect Salmonella with fluorescent microscopic imaging. This new nanoscale biosensor will be able to detect other foodborne pathogenic bacteria for food safety and security applications.