Submitted to: American Society for Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2008
Publication Date: 5/22/2008
Citation: Dowd, S.E. 2008. Salmonella enterica Typhimurium DT104 challenge of pigs using an ileal loop surgical model indicate early host responses involve IL-27 controlled INF pathway [abstract]. 108th American Society for Microbiology Annual Meeting. Abstract No. P-233.
Technical Abstract: Understanding the early responses of the immune system following infection with Salmonella is necessary in the search for management practices that might improve the health of animals and reduce the economic impact of this pathogen. Using a ligated ileal loop pig model, we challenged with Salmonella enterica DT104. Two hours post challenge we recovered ileocecal lymph nodes directly associated with the ligation. Using a 12K custom swine microarray we compared the expression of pigs infected with Salmonella to uninfected controls. Five separate interferon response genes were induced suggesting a type I immune response was initiated. The most highly induced genes (FDR < 5%) is a previously undescribed inflammatory response protein 6 (IRG6). Also induced were IL-27, CD72, Galactin, p21, CSF3R, GADD45B, and IL15RA which further clarify a type I interferon response. Only 5 genes were found to be repressed as part of this early response to Salmonella exposure. These included meox2, ankyrin 2, myomesin 2, and cytochrome P450. This study suggests that early induction of IL-27 promotes a type I interferon response. This is the first report suggesting that IL-27 in pigs modulates a type I IFN response to Salmonella enteric DT104.