|Ray, Jeffery - Jeff|
Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/25/2008
Publication Date: 4/8/2008
Citation: Seversike, T.M., Ray, J.D., Shultz, J.L., Purcell, L.C. 2008. Soybean Molecular Linkage Group B1 Cooresponds to Classical Linkage Group 16 Based on Map Location of the 1f2 Gene. Journal of Theoretical and Applied Genetics. 117:143-147 Interpretive Summary: In soybean there is a 7-leaf trait controlled by a single recessive gene as compared to the more normal 3-leaf trait. In classical genetics the 7-leaf trait is closely associated with dense pubescence. However, neither of these classical traits have been placed on the molecular map of soybean. As a result of this research these traits were determined to be located on the molecular linkage group B1 of soybean. These represent the only classical traits mapped to molecular linkage group B1.
Technical Abstract: The 7-leaflet character of soybean [Glycine max L. (Merr.)] is a single recessive trait conditioned by the lf2 gene. The lf2 gene is located on linkage group (LG) 16 of the classical soybean genetic map, but it has not been placed on the molecular map. The objective of this research was to identify the location of the lf2 gene on the soybean molecular map using simple sequence repeat (SSR) markers. A backcross breeding method was used to create 3- and 7-leaflet near-isogenic lines in genetic backgrounds of Traill, MN1401, and MN1801. Eight mapping populations were derived from eight single heterozygous Lf2 lf2 plants. A total of 482 SSR markers that covered approximately every 10 to 20 cM of all soybean molecular linkage groups were used to screen the mapping populations for polymorphisms. For the 115 SSR that were identified as polymorphic, possible linkage between the lf2 trait and the polymorphic SSR markers was determined. One SSR marker from LG B1, Sat_272, was linked (LOD > 4.0) to the lf2 gene in the Traill and MN1401 derived populations with map distances ranging from 2.77 to 11.2 cM. Two additional markers (a SSR, Sat_270 and a SNP, A588c) located on LG B1 were also polymorphic and identified as linked to the lf2 trait in one of the populations. This research was successful in mapping the lf2 gene to LG B1 of the soybean molecular map and therefore provides the molecular location of classical LG 16.