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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #216119
Title: Genetic analyses with oat-maize addition and radiation hybrid lines

Author
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item Rines, Howard

Submitted to: Maize Handbook
Publication Type: Book / Chapter
Publication Acceptance Date: 3/15/2008
Publication Date: 5/1/2009
Citation: Phillips, R.L., Rines, H.W. 2009. Genetic Analyses with Oat-Maize Addition and Radiation Hybrid Lines. In: Bennetzen, J.L., Hake, S., editors. Handbook of Maize: Genetics and Genomics. Volume II. New York, NY: Springer Science + Business Media, LLC. p. 523-538.

Interpretive Summary:

Technical Abstract: Oat-maize addition lines, with individual maize (Zea mays L.) chromosomes added to the oat (Avena sativa L.) genome via wide hybridization and embryo rescue, simplify the maize genome by 10-fold. Radiation hybrids have less than a complete maize chromosome in an oat genomic background, derived through gamma irradiation of monosomic addition lines. Maize genes and gene families can be readily located to their respective physical chromosome or chromosome segment by rapid, high-throughput, and straight-forward PCR experiments. Polymorphisms are not required to map a maize DNA fragment with this system. Numerous non-mapping uses of these lines currently underway are described. Seed and/or DNA of the addition lines in various oat and maize backgrounds are available as well as approximately 650 radiation hybrid lines.