|Dowd, Michael - Mike|
Submitted to: Anticancer Research
Publication Type: Peer reviewed journal
Publication Acceptance Date: 11/13/2006
Publication Date: 1/31/2007
Citation: Ye, W., Chang, H-L., Wang, L-S. Huang, Y-W., Shu, S., Dowd, M.K., Wan, P.J., Sugimoto, Y., Lin, Y. 2007. Modulation of multidrug resistance gene expression in human breast cancer cells by (-)-gossypol-enriched cottonseed oil. Anticancer Research. 27:107-116. Interpretive Summary: Experiments were conducted with (-)-gossypol-enriched cottonseed oil to demonstrate the potential of (-)-gossypol to inhibit drug resistant cancer cell lines. Addition of 0.05% of cottonseed oil to various therapeutic agents inhibited the growth of MCF-7/adr and PCHBCEC cells. The results will be of interest to researchers developing cancer therapies.
Technical Abstract: P-glycoprotein, the product of the multidrug resistance 1 gene, acts as an efflux pump and prevents sufficient intracellular accumulation of several anticancer agents. Thus, it plays a major role in multidrug cancer resistance. Using the non-radioactive cell proliferation MTS assay, none of three anticancer drug agents (Tamoxifen, ICI 182 780, and Adriamycin) inhibited MCF-7/adr (MDR human breast cancer) cells and only Adriamycin has a small inhibitory effect against PCHBCEC (primary cultured human breast cancer epithelial) cells. These same agents with 0.05% (-)-gossypol enriched cottonseed oil significantly inhibited MCF-7/adr cell growth by between 23 and 34%. For PCHBCE cells, the addition of (-)-gossypol enriched oil inhibited the cells by between 71 and 94%. The results demonstrate the multidrug-reversing potential of (-)-gossypol enriched cottonseed oil.