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United States Department of Agriculture

Agricultural Research Service


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Title: Effect of selected water temperatures used in Mycoplasma gallisepticum vaccine reconstitution on titer at selected time intervals)

item Branton, Scott
item Leigh, Spencer
item Roush, William
item Purswell, Joseph
item Olanrewaju, Hammed
item Collier, Stephanie

Submitted to: Avian Diseases
Publication Type: Peer reviewed journal
Publication Acceptance Date: 2/18/2008
Publication Date: 6/25/2008
Citation: Branton, S.L., Leigh, S.A., Roush, W.B., Purswell, J.L., Olanrewaju, H.A., Collier, S.D. 2008. Effect of selected water temperatures used in Mycoplasma gallisepticum vaccine reconstitution on color change units at selected time intervals. Avian Diseases. 52:291-296.

Interpretive Summary: Over the past 15 years it has become standard practice in the commercial table egg industry to vaccinate layer chickens with one of three commercially available live Mycoplasma gallisepticum (MG) vaccines in order to offset the loss of approximately 16 eggs/hen attributable to field strain MG infection. The vaccinal organisms are very sensitive to temperature, pH and osmolarity and with the cost of the vaccine alone at $1900/house of 75,000 birds, optimization of vaccine application parameters are critical. The present study was conducted to determine the effect of cool water (40 F) versus room temperature water (72 F) versus relatively warm water (90 F) on viability of each of the three commercially available live MG vaccines. The results of the study demonstrate that the cooler (40 F) water should be used to both reconstitute and dilute live MG vaccines in order to maintain and prolong the viability of the vaccinal organisms.

Technical Abstract: Numerous methods are currently used throughout the poultry industry for the administration of vaccines. Each utilizes water for vaccine reconstitution and/or administration, including two of the three commercially available live Mycoplasma gallisepticum (MG) vaccines. Selected water temperatures were used to reconstitute and/or dilute the three commercially available live MG vaccines. Water temperatures included 4 C, 22 C (room temp.), and 32 C and titer (color change units [CCU]) was recorded at four time intervals, at point of reconstitution (time 0), 15, 30 and 60 minutes post-reconstitution of the vaccines (time periods 15, 30 and 60, respectively. Results for F strain MG (FMG) vaccine showed significant decreases in titer from time 0 to time 15 for the 22 C and 32 C water temperatures but no significant decrease for any time period for FMG reconstituted with 4 C water. For 6/85 strain MG no significant difference in titer was noted for any of four time periods within any of the three water temperatures. For ts-11 strain MG a significant decrease was observed in titer at each of the four post-dilution time periods when diluted with 32 C water. There was no significant decrease in titer at any time period for ts-11 MG vaccine when diluted with either 4 C or 22 C water.

Last Modified: 8/24/2016
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