Submitted to: Proceedings of the Rocky Mountain Conference of Entomologists
Publication Type: Abstract only
Publication Acceptance Date: 8/23/2007
Publication Date: 8/23/2007
Citation: Reeves, W.K. Molecular Studies of Fly-Borne Pathogens. Rocky Mountain Conference of Entomologists, Woodland Park, CO. July 29 - August 2, 2007. Interpretive Summary:
Technical Abstract: Vector-borne diseases are among the most significant threats to agriculture and human health. Mosquitoes are the most significant vectors of disease, but other biting and blood feeding flies such as black flies (Simuliidae), keds (Hippoboscidae), bot flies (Oestridae), and stable flies (Muscidae) are potential vectors of pathogens. I discuss the applications of molecular diagnostic techniques such as immunoassays, PCR, and CAPM on these flies for the detection of the agents of viral, bacterial, and prions diseases. Unexpected results from some of these assays included the amplification of non-target DNA and the detection of host proteins that confounded immunoassays.