Submitted to: Conference Research Workers Disease Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/30/2007
Publication Date: 12/2/2007
Citation: Lange, A., Greiser-Wilke, I., Bendfeldt, S., Miller, L.C., Neill, J.D. 2007. Determination of the cytokine expression profile after infection of PK-15 Porcine cells with classical swine fever virus [abstract]. Conference of Research Workers in Animal Diseases Annual Meeting. Paper No. 137P. p. 118.
Technical Abstract: Classical swine fever (CSF) is caused by the Classical swine fever virus (CSFV), a member of the genus Pestivirus within the family Flaviviridae. A highly contagious disease of domestic pigs and wild boars worldwide it causes serious losses to the pig industry. The virulence of CSF viruses is strain dependent. The mechanisms of CSFV pathogenesis is as yet unknown. It is hypothesized that differences in cytokine expression may result in differences in virulences. In a preliminary study, interferon beta declined while TNF alpha and IL6 showed no change in CSFV-infected cells. The aim of the current study was to get an overview of mechanisms involved in the pathogenicity of CSFV in the host cell using a powerful technique known as the serial analysis of gene expression (SAGE). In initial experiments, SAGE was employed to examine the global gene expression changes between CSFV infected and non infected PK-15 cells. In particular, the cytokine gene expression profile was analyzed. In the second phase of this study, high and low virulence CSFV were compared using SAGE libraries generated at 2 time points from non-infected PK-15 cells, 2 time points from PK-15 cells infected with the high virulent CSFV strain Kozlow and 1 time point from PK-15 cells infected with a low virulence CSFV strain.