|Smith, C wayne|
Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract only
Publication Acceptance Date: 3/7/2006
Publication Date: 3/7/2006
Citation: Brake, D.K., Robker, R.L., Smith, E.O., Smith, C.W. 2006. Diet-induced increases in ICAM-1, CD11c, CD34 in adipose tissue of male mice [abstract]. Federation of American Societies for Experimental Biology Journal. 20(5):A1038. Interpretive Summary:
Technical Abstract: Obesity has been linked to cardiovascular disease, hypertension, hypercholesterolemia, insulin resistance and metabolic syndrome with elevated markers of systemic inflammation. Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane adhesion molecule involved in leukocyte migration to sites of inflammation. In human obesity, abdominal fat deposition is positively correlated with elevations in the soluble form of ICAM-1 (sICAM-1). An obese state has also been correlated with increased macrophage infiltration into mouse adipose tissue. Here we investigate adipose tissue production and transcriptional regulation of ICAM-1 in a mouse model of obesity induced by a 21% milkfat, high cholesterol diet. ICAM-1 in serum and adipose tissue was analysed by ELISA, northern blot, real-time quantitative PCR, and flow cytometry. After 6 months on the high fat diet sICAM-1 levels significantly correlated with body and abdominal fat pad weights. Northern blots from adipose tissue showed significantly higher levels of ICAM-1 mRNA in males than females. After 3 weeks on the high fat diet there was an adipose tissue-specific increase in mRNA for ICAM-1, IL-6, and MCP-1 in male but not female mice. Analysis of the stromal vascular fraction of male adipose tissue revealed CD11b negative cells with increased surface ICAM-1 and CD34. We also found two populations of F4/80+, CD11b+, and ICAM-1+ cells, one of which was positive for CD14 and CD11c and significantly increased in response to a high fat diet. These results indicate that 3 weeks of a high fat diet induce significant increases in pro-inflammatory factors in adipose tissue of male mice that may represent links between obesity and its associated inflammatory-like complications.