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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Composition and Methods Development Laboratory » Research » Publications at this Location » Publication #214938

Title: Phenolic Compounds Analysis in Foods and Dietary Supplements is not Same by Different Sample Preparation Procedures

item Luthria, Devanand - Dave

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/14/2007
Publication Date: 10/9/2007
Citation: Luthria, D.L. 2007. Phenolic Compounds Analysis in Foods and Dietary Supplements is not Same by Different Sample Preparation Procedures. Fav Health 2007, October 9-13, 2007, Houston, TX.

Interpretive Summary:

Technical Abstract: Recent epidemiological studies suggest a positive correlation between diets rich in fruits and vegetables and a reduce incidence of chronic diseases. This beneficial effect is partially attributed to secondary phenolic phytochemicals. More than 8,000 different phenolic phytochemicals belonging to different subgroups have been identified. The large number of phenolic compounds, their structural diversity and their interaction with sample matrix present a considerable challenge to develop an efficient procedure for optimum extraction and accurate analysis form different plant matrices. This paper illustrates the issues related to extraction of phenolic compounds as described in peer-reviewed literature. It discusses the importance of optimizing sample preparation procedures for accurate estimation of phenolic compounds from foods (eggplant, soybean, and parsley) and dietary supplements (Black Cohosh and Gingko Biloba). Comparison of current and classical extraction procedures for the extraction of phenolic phytochemicals will also be presented. Influence of different extraction parameters such as extraction solvent composition, particle size, temperature, solid-to-solvent ratio, pressure, and number of cycles will be presented. A systematic approach for optimum extraction of phenolic phytochemicals from different plant matrices will also be discussed. Accurate quantitation of bioactive phenolic phytochemicals will allow researchers to provide better guidelines on dietary intake levels necessary to achieve the desired health-beneficial effects.