|O Hearn, Emily|
Submitted to: American Society for Virology Meeting
Publication Type: Proceedings
Publication Acceptance Date: 4/1/2007
Publication Date: 7/18/2007
Citation: Wilson, W.C., O Hearn, E.S., Roth, C., Lenhoff, R. 2007. Genetic analysis of the NS1 and NS3 genes from the prototype serotype of Bluetongue and Epizootic Hemorrhagic Disease Viruses. American Society for Virology Meeting. Corvallis, OR. July 14-18, 2007.
Technical Abstract: Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are arthropod-borne viruses of significant animal agriculture importance. Clinical disease caused by BTV is most commonly observed in sheep and some wild ruminants; however, the recent outbreak in European Union has resulted in severe disease in cattle. EHDV is usually associated with clinical disease in white-tailed deer, but also has been recently reported in cattle. Diagnostic test that differentiate these viruses are needed because these viruses can be clinically similar. Recently, real-time RT-PCR have been developed to detect all 24 serotypes of BTV, however, no real-time RT-PCR has been reported to detect all 8 serotypes of EHDV. We sequenced the complete NS1 and NS3 genes for the 19 BTV and 6 EHDV protype strains that were not available to facilitate the optimal design of a multiplex real-time PCR. These two genes are primary targets for diagnostic RT-PCR because they conserved and highly expressed in their vertebrate and invertebrate hosts, respectively. In this report we describe the phylogenetic analysis of these sequences in relation to the NS! and NS3 gene sequences available to Genbank. These genes were more diverse than expected based on previously published data. The viruses do display a tendency to group temporally and geographically but not by serotype. This sequence data has been used to design optimal RT-PCR signatues for these viruses. The design and development of multiplex real-time RT-PCR will be described in a separate presentation.