|HEIDENREICH, M. - University Of Wisconsin|
|ZHIQIANG, AN - University Of Wisconsin|
Submitted to: Fungal Genetics Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/4/2008
Publication Date: 12/4/2008
Citation: Heidenreich, M.L., Budde, A.D., Zhiqiang, A., Leong, S.A. 2008. Disruption of a yeast ADE6 Homolog in Ustilago maydis. Fungal Genetics Reports. 55:40-43.
Interpretive Summary: Ustilago maydis is a useful model for fungal plant pathogenesis. Recently, the entire DNA sequence of the fungal chromosomes has been determined. In this paper we have characterized and described two genes in this DNA sequence that are next to one another. These genes were not previously annotated in the fungal genome. One gene helps the fungus acquire iron from the environment and the other is essential to fungal growth and development. Our work contributes to a more complete understanding of this fungal genome.
Technical Abstract: Ustilago maydis secretes iron-binding compounds during times of iron depletion. A putative homolog of the Sacharromyces cereviseae ADE6 and Escherichia coli purL genes was identified near a multigenic complex, which contains two genes sid1 and sid2 involved in a siderophore biosynthetic pathway. The putative ADE6 homolog was mutated by targeted gene disruption. The resulting mutant strains demonstrated a requirement for exogenous adenine, indicating that the U. maydis ade6 homolog is required for purine biosynthesis.