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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #213101

Title: Cross Species Amplification of Microsatellite Markers in Fragaria

item Bassil, Nahla
item Hummer, Kim
item Slovin, Janet

Submitted to: North American Strawberry Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 6/28/2007
Publication Date: 4/20/2008
Citation: Njuguna, W., Bassil, N.V., Hummer, K.E., Slovin, J.P. 2008. Cross Species Amplification of Microsatellite Markers in Fragaria. North American Strawberry Conference Proceedings 2007. p. 122-123.

Interpretive Summary: Molecular markers are a preferred method for plant identity analysis. We use a DNA-based technique called simple sequence repeats (SSR). These markers can distinguish between individual plants of one species. The objectives of this study were to develop these markers for strawberries and to determine if a marker for one species could be used in other species. We isolated 19 markers from a European strawberry called ‘Yellow Wonder’. Fourteen of them could be used in thirteen other strawberries species. The markers were also useful for comparing the common hybrid strawberry and its parents. These markers will be useful in studies that examine the origin and family history of strawberries as well as the locations of important genes on the chromosomes.

Technical Abstract: Microsatellites or simple sequence repeats (SSRs) are one of the most preferred DNA-based tools for variety identification and linkage mapping. The objective of this study was to develop expressed sequence tag (EST)-SSR primers in Fragaria and determine their cross species amplification in fourteen species. These species included eight diploids, one tetraploid, one hexaploid and four octoploids. Nineteen EST-SSR primer pairs were designed from sequences of Fragaria vesca L. cv. Yellow Wonder. Fourteen of these cross-amplified in thirteen Fragaria species. Transferability ranged from 71% in the diploid species F. gracilis Losinsk, F. iinumae Makino, F. nilgerrensis Schltdl. Ex. J. Gay and F. nipponica Makino to 100% in the octoploid domesticated strawberry F. ×ananassa Duschesne and its progenitors. High cross-species transference of these EST-SSRs in Fragaria will improve the current linkage map and facilitate comparative studies.