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United States Department of Agriculture

Agricultural Research Service

Title: Biomarkers of CD4+ CTL cell Mediated Immunity to Tuberculosis

item Endsley, Janice
item Hogg, Alison
item Shell, Lis
item Mcalauy, Martin
item Scherer, Charles
item Coffey, Tracey
item Howard, Chris
item Nonnecke, Brian
item Waters, Wade
item Estes, D

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/18/2007
Publication Date: 5/18/2007
Citation: Endsley, J.J., Hogg, A., Shell, L., Mcalauy, M., Scherer, C.C., Coffey, T., Howard, C., Nonnecke, B.J., Waters, W.R., Estes, D.M. 2007. Biomarkers of CD4+ CTL cell Mediated Immunity to Tuberculosis [abstract]. American Association of Imunologists. p. 43.49.

Interpretive Summary:

Technical Abstract: The immune responses mediated by interactions between T-lymphocyte subsets and mycobacteria-infected macrophages are critical for control of tuberculosis. In these studies, the bovine model was used to characterize the cytolytic and mycobactericidal CD4+ T cell response induced by BCG vaccination. Antigenic stimulation of CD4+ T-cells from BCG vaccinated cattle induced expression of perforin and IFNgamma in cells expressing a CD45RA-, CD45RO+, and CD62L+ cell surface phenotype. Antigen specific enhancement of granulysin, IFNgamma, perforin, IL-4, IL-13, and IL-21 mRNA expression was detected and not detected for IL-2, IL-6, IL-10, IL-15, TNF {alpha}<>, FasL, and CD40L. Following antigenic stimulation, CD4+ T cells from BCG vaccinated animals contributed to reduction of intracellular BCG in infected macrophages. These results demonstrate that vaccination with BCG induces a subpopulation of mycobacteria-specific CD4+ T cells that are characterized by the expression of a cell-surface memory phenotype, enhanced expression of mycobactericidal molecules, and anti-mycobacterial activity against intracellular M. bovis. This work was supported by the NIAID Fellowship for Training in Emerging and Re-emerging Infectious Disease and the Sealy Center for Vaccine Development, UTMB, the Texas-United Kingdom Collaborative Research Initiative, and the Institute for Animal Health by DEFRA and the BBSRC, UK.

Last Modified: 10/15/2017
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