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United States Department of Agriculture

Agricultural Research Service

Title: Bo-lysin: A Potential Candidate as a biomarker of Protection after Vaccination against Tuberculosis

item Scherer, Charles
item Endsley, Janice
item Aguiar, Juliana
item Waters, Wade
item Estes, D

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/18/2007
Publication Date: 5/18/2007
Citation: Scherer, C.F., Endsley, J.J., Aguiar, J.B., Waters, W.R., Estes, D.M. 2007. Bo-lysin: A Potential Candidate as a biomarker of Protection after Vaccination against Tuberculosis [abstract]. American Association of Immunologists. p. 47.39.

Interpretive Summary:

Technical Abstract: Tuberculosis (TB) is still a major health problem worldwide. A Th1 type response with release of IFN {gamma}< and cytotoxic granules such as granulysin and perforin, play a major role in the disease. Measurements of protection after TB vaccination include IFN {gamma}<> measures and T cell proliferation assays, which frequently fail to correlate well with protection. The lack of better vaccines than BCG and good correlates of protection demands that new biomarkers are found. A bovine homologue of granulysin (bo-lysin), cloned by our group, may be a good candidate as a biomarker molecule for TB vaccination. In the present study we examined the kinetics of bo-lysin, compared to perforin, IFN {gamma}<> and Fas-L in vitro, after different stimulation conditions in different bovine T cell populations. Gene expression profile showed bo-lysin and IFN {gamma}<> expression increase in an equal manner, with IFN {gamma}<>showing up as early as 4 hours of stimulation and Bo-lysin at 24 hours, in BCG vaccinated animals. No expression was detected in non-vaccinated animals.Detection of IFN {gamma}<> and perforin by flow cytometry showed increasing percentages of antigen specific perforin+ or IFN {gamma}<> + CD4+ and perforin+ or IFN {gamma}<> +CD8+ T cells in BCG vaccinated animals, but none were observed in non-vaccinated animals. Results show that granulysin is a good candidate as a biomarker of protection after vaccination against TB.

Last Modified: 09/20/2017
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