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Title: Bacteriocins control chicken colonization while probiotic bacteria are ineffective at reducing Campylobacter jejuni

Author
item SVETOCH, E - ST RES CTR RUSSIA
item ERUSLANOV, B - ST RES CTR RUSSIA
item POKHILENKO, V - ST RES CTR RUSSIA
item KOVALEV, Y - ST RES CTR RUSSIA
item VOLODINA, L - ST RES CTR RUSSIA
item PERELYGIN, V - ST RES CTR RUSSIA
item MITSEVICH, E - ST RES CTR RUSSIA
item MITSEVICH, I - ST RES CTR RUSSIA
item BORZENKOV, V - ST RES CTR RUSSIA
item LEVCHUK, V - ST RES CTR RUSSIA
item SVETOCH, O - ST RES CTR RUSSIA
item KUDRIAVTSEVA, T - ST RES CTR RUSSIA
item Stern, Norman

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 6/8/2007
Publication Date: 9/2/2007
Citation: Svetoch, E.A., Eruslanov, B.V., Pokhilenko, V.D., Kovalev, Y.N., Volodina, L.I., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Borzenkov, V.N., Levchuk, V.P., Svetoch, O.E., Kudriavtseva, T.Y., Stern, N.J. 2007. Bacteriocins control chicken colonization while probiotic bacteria are ineffective at reducing Campylobacter jejuni. Campylobacter Helicobacter and Related Organisms International Workshop.

Interpretive Summary:

Technical Abstract: Broilers chickens are widely considered an important source for human exposure to Campylobacter jejuni. We sought to intervene in C. jejuni colonization by using a probiotic approach. Isolates from chicken intestine were screened for C. jejuni inhibition. These isolates were live-fed to treat chicks before or after C. jejuni challenge. When C. jejuni challenges approached the chick colonization dose-50%, the prophylactic probiotic treatments were effective. At higher challenges probiotic treatments failed to control C. jejuni colonization. Unless the treatment totally eliminated the agent, as birds further aged, C. jejuni numbers soon approached those in untreated control birds. Two of our most promising probiotic isolates, Lactobacillus salivarius NRRL B-30514 and Paenibacillus polymyxa NRRL B-30509, were further studied to control C. jejuni in chickens. When 250 mg of purified bacteriocins from these probiotic strains per Kg feed were fed to C. jejuni colonized chickens, colonization was reduced by at least one-million fold. Probiotic bacterial cultures were, at best, only modestly effective in controlling C. jejuni in chickens. However, oral administration of bacteriocins in mature birds almost totally eliminated C. jejuni.