Author
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Reed, Barbara |
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Submitted to: Plant Cryopreservation, A Practical Guide
Publication Type: Book / Chapter Publication Acceptance Date: 5/1/2007 Publication Date: 11/12/2007 Citation: Reed, B.M. 2007. Cryopreservation of Temperate Berry Crops. In: Reed, B.M. Plant Cryopreservation, A Practical Guide. New York, NY:Springer. p. 333-364. Interpretive Summary: Preservation of clonal fruit crops requires vegetative propagation in most cases because seeds do not produce identical plants. Cryopreservation in liquid nitrogen (-320 °C) is considered an ideal method for long-term germplasm storage. Most of the existing cryopreservation techniques are effective for temperate berry crops such as blackberries, strawberries and grapes. The availability of many techniques provides options for germplasm curators who wish to use cryopreservation to back up plant collections, or for researchers who need to hold important plant materials for future use. This chapter describes the current techniques available for temperate berry crops and includes eight step-by-step protocols for direct use. Technical Abstract: Preservation of clonal fruit crops requires vegetative propagation. Cryopreservation in liquid nitrogen is considered an ideal method for long-term germplasm storage. Most of the existing cryopreservation techniques are effective for temperate berry crops. The availability of many techniques provides options for germplasm curators who wish to use cryopreservation to back up plant collections, or for researchers who need to hold important plant materials for future use. This chapter describes the current techniques available for temperate berry crops and includes eight step-by-step protocols for direct use. |