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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #210559

Title: List of Predicted Simple Sequence Repeats from Sugar Beet GenBank Accessions

Author
item Mcgrath, J Mitchell - Mitch

Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Research Notes
Publication Acceptance Date: 5/30/2007
Publication Date: 6/19/2007
Citation: McGrath, J.M. 2007. List of Predicted Simple Sequence Repeats from Sugar Beet GenBank Accessions. Annual Beet Sugar Development Foundation Research Report. p. D28-D77.

Interpretive Summary:

Technical Abstract: Beta vulgaris ESTs from GenBank as of January 2005 collapsed into 13,618 unique clusters (4,023 Tentative Consensus sequences, 9,595 singletons), and 35% were contributed via work partially supported through the BSDF. These sequences were parsed through SSR-Primer software for discovering potential SSR genetic markers, and 2,175 potential SSR markers were identified. 23,067 paired BAC end sequences were determined as of 8/30/2006, and these sequences were parsed for SSR motifs in a similar manner as the ESTs, yielding 457 potential genetic markers. An additional set of 2,523 sequences were obtained via a pilot methyl-filtration project, and of these 44 returned a potential SSR marker. A total of 2,676 potential SSR markers were identified, and mapping these to linkage groups is continuing.