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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #210557

Title: Understanding Transcription Factors in Sugar Beets: Genetic and Physical Mapping, Differential Expression, and Conservation Between Related Germplasm

Author
item Kim, Robert
item Mcgrath, J Mitchell - Mitch

Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Research Notes
Publication Acceptance Date: 5/30/2007
Publication Date: 6/19/2007
Citation: Kim, R., McGrath, J.M. 2007. Understanding Transcription Factors in Sugar Beets: Genetic and Physical Mapping, Differential Expression, and Conservation Between Related Germplasm. Annual Beet Sugar Development Foundation Research Report. p. D15-D25.

Interpretive Summary:

Technical Abstract: Transcription factors control all biological processes at the cellular level, but their role in sugar beets is still widely unknown. In order to develop a greater understanding, 47 primer pairs were designed around expressed tag sequences (ESTs) whose putative functions are various transcription factors. These primers were used to try and discover genetic and physical markers, examine expression in six cDNA libraries, and examine conservation between related germplasms. No genetic or physical markers were mapped on an existing map, but five primer pairs were linked to each other to form an island that can be placed on the genetic map as more markers are discovered. Expression data matched expected data for three transcription factors based on their known function in other organisms, indicating similar biological roles in sugar beet as their know functions in other plants. The conservation data also confirmed the existing phylogeny of the eight germplasm tested.