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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #210467

Title: Molecular Diversity of Gossypium herbaceum L. and G. arboreum L.

item Sacks, Erik
item Ulloa, Mauricio

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: 4/10/2007
Publication Date: 5/24/2007
Citation: Kantartzi, S., Sacks, E.J., Ulloa, M., Stewart, J. 2007. Molecular Diversity of Gossyplum Herbaceum L. and G. Arboreum L.. National Cotton Council Beltwide Cotton Conference. P 233

Interpretive Summary:

Technical Abstract: The diploid Asiatic cotton species (Gossypium herbaceum L. and G. arboreum L.) are a valuable source of genetic variation in modern cotton improvement. In this study, the genetic diversity of selected G. herbaceum and G. arboreum accessions collected from different regions all around the world according to their passport data was evaluated by microsatellite (simple sequence repeats, SSRs) analysis. Of the 96 SSR primer pairs applied to the 15 materials, 17 detected monomorphic products in each sample and 38 produced very faint or undecipherable bands. The remaining 41 primer pairs yielded totally 96 polymorphic bands. The number of polymorphic fragments generated by each primer pair ranged from 2 to 6, with an average of 2.34. The genetic similarity coefficients among accessions ranged from 0.52 to 0.79 with an average of 0.71 in G. herbaceum and 0.50 to 0.88 with an average of 0.72 in G. arboreum. The next objective of our work is to evaluate SSR primer sets from different series for polymorphisms and additional accessions of both A genome species in order to determine the putative diversity as extensively as possible.