Submitted to: Book Chapter
Publication Type: Book / chapter
Publication Acceptance Date: 4/30/2007
Publication Date: 8/30/2008
Citation: Kim, H., Bae, J., Hou, C.T. 2008. Production of biologically active hydroxy fatty acids by Pseudomonas aeruginosa PR3. In: Hou, C.T., editor. Biocatalysis and Bioenergy. New York, NY:John Wiley & Sons. p. 557-570. Interpretive Summary:
Technical Abstract: Hydroxy fatty acids (HFA) have gained important attentions because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. Pseudomonas aeruginosa (PR3) had been reported to produce mono-, di-, and tri-hydroxy fatty acids from different unsaturated fatty acids. Of these, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced with high yield from oleic acid by PR3. Up to now, the substrates used for microbial HFA production were free fatty acids. However, triacylglyceride, specifically triolein is possible to be utilized as a substrate by microbial enzyme system involved in HFA production from oleic acid. Now we used triolein as a substrate and firstly report that triolein could be efficiently utilized by PR3 to produce DOD. Triolein was first hydrolyzed into oleic acid by the triolein-induced lipase and then the released oleic acid was converted to DOD by PR3. Results from this study demonstrated that natural vegetable oils, without being intentionally hydrolyzed, could be used as efficient substrate for the microbial production of value-added hydroxy fatty acids. We also found that crude extracts of bioconverted linoleic acid using P. aeruginosa PR3 showed antibacterial activity against several Gram (+) bacteria and one Gram (-) bacteria.