Submitted to: Fungal Genetics Conference/Asilomar
Publication Type: Abstract only
Publication Acceptance Date: 3/25/2007
Publication Date: 3/25/2007
Citation: Schonig, B., Schmeinck, A., Brown, D.W., Tudzynski, B. 2007. The role of MeaB, NMR and AreB as antagonists of AreA in Fusarium fujikuroi [abstract]. Fungal Genetics Conference/Asilomar. p. 311. Interpretive Summary:
Technical Abstract: The rice pathogen Fusarium fujikuroi produces the phytohormones gibberellins (GAs), and the red polyketide pigment bikaverin. The production of both metabolites is subject to nitrogen metabolite repression (NMR): the central regulator of nitrogen repression, AreA, has been shown to directly activate the transcription of 6 out of 7 GA biosynthetic genes under nitrogen limitation conditions, whereas the effect on bikaverin seems to be indirect. Therefore, AreA does not only regulate genes involved in primary metabolism but also genes for secondary metabolites. To get a better overview on AreA target genes in total, we used microarrays spotted with 16,000 EST clones of the close relative F. verticillioides and compared expression of genes of the wild type strain and the areA deletion mutant under nitrogen sufficient and starvation conditions. For better understanding of the action of AreA, we are searching for other key regulators which might interact with AreA, such as the putative negative regulators Nmr, MeaB and AreB, whose homologues were shown to be involved in nitrogen metabolite repression in Aspergillus nidulans.